flow cytometry sub-micron particle size reference kit (Thermo Fisher)
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flow cytometry sub-micron particle size reference kit
Flow Cytometry Sub Micron Particle Size Reference Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry+sub-micron+particle+size+reference+kit/flow+cytometry+sub+micron+particle+size+reference+kit/bio_rxiv__2025__07__11__664236-295-4-11
Average 90 stars, based on 1 article reviews
Flow Cytometry Sub Micron Particle Size Reference Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/flow+cytometry+sub-micron+particle+size+reference+kit/flow+cytometry+sub+micron+particle+size+reference+kit/bio_rxiv__2025__07__11__664236-295-4-11
Average 90 stars, based on 1 article reviews
flow cytometry sub-micron particle size reference kit - by Bioz Stars,
2026-10
90/100 stars
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1) Product Images from "Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts"
Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts
Journal: bioRxiv
doi: 10.1101/2025.07.11.664236
Figure Legend Snippet: Validation of nano particle flow cytometry methodology. A. polystyrene microspheres of the indicated size. B . EVs were enriched from lean (normal chow) or obese (12 weeks high-fat diet) mouse plasma (2µl), with size exclusion chromatography (SEC) and analyzed by flow cytometry. The SSC detector was calibrated to display EV diameter C. CFSE stained EVs from lean and obese mice processed as in B. D. quantification of C E. A representative experiment where plasma is labeled with a cocktail of antibodies for EVs (CD63, CD81, ITGB1), or lipoproteins (APOE, APOB). EVs were enriched with SEC before flow analysis. F. Mouse plasma stained with an adipocyte marker mix (perilipin 1 and adiponectin). Plasma was harvested from either wild type mice or adipocyte-specific PPARγ knockout mice (Adipo-PPARγKO). G . Plasma stained from lean or obese (12 weeks high-fat diet). Total EVs that stain with the adipocyte marker mix (AdipoEVs) and EVs that co-stain with the both the adipocyte and EV marker mixes (Adipo/EV maker). H. Mitochondria isolated from mouse subcutaneous adipose tissue and stained with mitochondrial proteins: VDAC and COXIV or VDAC and TOM20. I. Plasma from mice fed a chow or high-fat diet (6 weeks) stained with markers for the indicated cell types. J. The percentage of EVs from each cell type in mouse plasma. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001
Techniques Used: Biomarker Discovery, Flow Cytometry, Clinical Proteomics, Size-exclusion Chromatography, Staining, Labeling, Marker, Knock-Out, Isolation
Figure Legend Snippet: Circulating adipocyte EVs and EV-mitos are increased with high-fat feeding early and persistently. A. Body weights for a cohort of WT male and female (N=6 for each sex and group). B. Nano particle flow cytometry for lipoprotein markers in mice plasma (APOB/E antibodies). Mice were on diets for 6 weeks. C-D Total EVs in plasma (CFSE labeled) were quantified at day 0 and day 1 of the feeding experiment ( C ) or at baseline, and 30 or 60 minutes after bleeding ( D ). Plasma as collected from male and female cohorts (N=6) via tail vein bleed over the indicated time course after diet initiation (chow or high-fat diet; HFD). E-L EVs were analyzed for total EVs (CFSE-stained; E-F ), adipocyte EVs (adipoEVs; APN/PLN1 + ; G-H ), adipocyte EV-mitos (adipoEV-mitos; APN/PLN1 + , VDAC + ; I-J ), and total mitochondria (VDAC + , K-L ). E, G, I and K are acute timepoints. F, H, J, L are chronic timepoints. Data are presented as mean ± s.e.m. * P < 0.05, *** P < 0.001.
Techniques Used: Flow Cytometry, Clinical Proteomics, Labeling, Staining
Figure Legend Snippet: AdipoEVs correlate with insulin resistance in people with obesity. Plasma samples from metabolically healthy lean (MHL), metabolically healthy obese (MHO) and metabolically unhealthy obese (MUO) patients were analyzed by nano flow cytometry for A. Lipoproteins (APOE/B + ), B. total EVs (CFSE + ), C. adipoEVs (APN/PLN1 + ), D. adipoEV-mitos (APN/PLN1 + , VDAC + ), E. total mitochondria (VDAC + ), F. Adipose tissue (AT) immune cell EVs (APN/PLN + , CD45 + ), G. total immune cell EVs (CD45 + ), or H. Endothelial cell (EC) EVs (CD31 + /CD45 - ). AdipoEVs correlations with I. % fat mass J. insulin sensitivity, K. hepatic insulin sensitivity index (HISI), L. homeostatic model assessment of insulin resistance (HOMA-IR), M. Intrahepatic triglyceride (IHTC) content. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001.
Techniques Used: Clinical Proteomics, Metabolic Labelling, Flow Cytometry
Figure Legend Snippet: Macrophage-mediated clearance of EVs is reduced with high-fat feeding. A. AdipoEVs (APN/PLN1 + ) correlate with total EVs (CFSE + ). B. Timeline of clodronate experiment. Mice were on a chow of high fat diet for 6 weeks at which point 5µl/gram mouse body weight of either control liposomes or clodronate liposomes were injected into mice at day 0 and day 3. Blood was sampled at the indicated timepoints. At day 4 of macrophage depletions and fed/fasted study was conducted. At day 6, mice were injected i.v. with purified plasma or adipocyte EVs labeled with CFSE for determination of EV clearance rates. Total EVs (CFSE + ; C ) and adipoEVs ( D ) were quantified at the indicated timepoints. At day 2 post clodronate injection plasma samples were analyzed for E. hepatocyte EVs (ASGR1 + /CD45 - ), F. endothelial cell (EC) EVs (CD31 + /CD45 - ), G. myocyte EVs (MHC + , CD45 + ). H. platelet EVs (CD41 + ), I. red blood cell (RBC) EVs (TER119 + ), or J . lipoproteins (APOE/B + ). K-M Mouse plasma EVs were harvested, labeled with CFSE, purified by SEC and injected retro-orbitally into mice on a chow or high fat diet (HFD) either treated with empty liposomes (Control) or clodronate liposomes (Clod). Tail blood was sampled at the indicated timepoints following CFSE + EV injection and the CFSE + EV remaining in the blood was quantified. N-O Mice treated with empty liposomes (Control) or clodronate liposomes were either ad libitum fed or fasted for 16 hours and refed for 3 hours. Plasma was sampled under each metabolic state and total EVs (CFSE; N ) and adipoEVs ( O ) were quantified. At day 7 of the experiments (4 days after the last clodronate injection) mice were euthanized. P. Macrophages in the eWAT and Q. monocytes in the blood were quantified by flow cytometry in mice treated with control liposomes (Cntrl) or clodronate liposomes (Clod). R. EV clearance assay for adipocyte-derived CFSE + EVs, as described in K-M. S. Blood monocyte uptake of adipocyte-derived CFSE + EVs at the indicated timepoints after EV injection by flow cytometry. Data are presented as mean ± s.e.m. * P < 0.05, ** P < 0.01, *** P < 0.001.
Techniques Used: Control, Liposomes, Injection, Purification, Clinical Proteomics, Labeling, Flow Cytometry, Derivative Assay
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Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. 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Article Snippet: The following reagents and chemicals were used: Injection:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Purification:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Derivative Assay:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Sonication:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Imaging:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Concentration Assay:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Binding Assay:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: Fluorescence:Article Title: Comparative analysis of the quality of platelet concentrates produced by apheresis procedures, platelet rich plasma, and buffy coat. Article Snippet: Funding information Scientific Research Project of Sichuan Health Committee, Grant/Award Number: 20PJ231 Abstract Background: Platelet concentrates (PCs) could be prepared using either whole-blood processes or apheresis instruments.. During collection, processing and storage, some biochemical and functional changes occur, which may result in quality reduction.. Quality evaluation of PCs may be helpful for the precise control of platelet (PLT) inventory to reduce the risk of refractoriness and adverse effects caused by platelet transfusion. Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study Article Snippet: For the established gating strategies of EV samples, we used Article Title: Proteomic profile of extracellular vesicles from plasma and CSF of multiple sclerosis patients reveals disease activity-associated EAAT2 Article Snippet: In particular, for the correct setting of the gate (based on the size of EVs) and the fluorescence parameters, fluorescent beads of variable size were used ( Article Title: Profiling of miRNAs Contained in Circulating Extracellular Vesicles and Associated with Sepsis Development in Burn Patients: A Proof-of-Concept Study. Article Snippet: For the established gating strategies of EV samples, we used Article Title: Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shifts Article Snippet: Parameters were optimized using Article Title: Innovative Methodology for Antimicrobial Susceptibility Determination in Mycoplasma Biofilms Article Snippet: To determine the specific size of the disrupted particles, a Article Title: Reduced circulating CD63 + extracellular vesicle levels associate with atherosclerosis in hypercholesterolaemic mice and humans Article Snippet: Measurements were carried out using the “slow” flow rate (10 μL/min) for 2 min and events were obtained from a previously optimized gate for EVs detection that was set up following a protocol outlined in [ , ] using Article Title: Comparative Analysis of Canonical Inflammasome Activation by Flow Cytometry, Imaging Flow Cytometry and High-Content Imaging. Article Snippet: The following reagents and chemicals were used: |